Journal: Frontiers in Neuroanatomy

Article Title: The identification and neurochemical characterization of central neurons that target parasympathetic preganglionic neurons involved in the regulation of choroidal blood flow in the rat eye using pseudorabies virus, immunolabeling and conventional pathway tracing methods

doi: 10.3389/fnana.2015.00065

Figure Lengend Snippet: Choroidal SSN is enriched in serotonergic terminals (A), and PRV+ SSN choroidal neurons (red) were seen to be contacted by serotonergic terminals (green) (B) . Moreover, choroidal SSN is enriched in 5HT2A serotonin receptors (C) .

Article Snippet: Antibodies against the following substances were used: nNOS (Santa Cruz SC-648, raised in rabbit, used at 1:200–1:800), calbindin (Sigma C9848, raised in mouse, used at 1:5000), or tyrosine hydroxylase (Immunostar, raised in mouse, used at 1:2000), the type-2 vesicular glutamate transporter (VGLUT2, Sigma V2514, raised in rabbit, used at 1:1000), serotonin (Immunostar, raised in rabbit, used at 1:10,000), the 5HT2A serotonin receptor (Immunostar, raised in rabbit, used at 1:1000), oxytocin (provided by Harold Gainer, NIH, raised in mouse, used at 1:500), and vasopressin (provided by Alan Robinson, retired, raised in rabbit, used at 1:20,000).

Techniques:

Journal: Respiratory Research

Article Title: Apoptosis of viral-infected airway epithelial cells limit viral production and is altered by corticosteroid exposure

doi: 10.1186/1465-9921-7-78

Figure Lengend Snippet: Representative images of airway epithelial immunostaining of Cam Hartley Guinea pigs Semi-quantitative scoring was utilized to determine the expression for DR4(A), DR5 (C) and p85-PARP (E) in immunohistochemically stained lung sections. Panels B, D and F were the isotype controls for the respective antibodies. Arrows indicate the stained epithelial cells. Scale bar represent 10 μm in panels A through F.

Article Snippet: Polyclonal rabbit anti-DR4 and -DR5 antibodies (Cell Sciences Inc, MA) and rabbit anti- PARP p85 fragment antibody (Promega, MA) were used along with normal Rabbit IgG as negative control to measure receptor expression and apoptosis respectively. p85-PARP antibody is specific for the p85 fragment of PARP generated by caspase cleavage and provides a reliable measure of in situ apoptosis [ ].

Techniques: Immunostaining, Expressing, Staining

Journal: Respiratory Research

Article Title: Apoptosis of viral-infected airway epithelial cells limit viral production and is altered by corticosteroid exposure

doi: 10.1186/1465-9921-7-78

Figure Lengend Snippet: Acutely infected GPTEC demonstrate apoptosis coordinate with DR4 and DR5 expression Semi-quantitative scoring was utilized to determine the expression of p85-PARP, DR4 and DR5 in the Guinea pig lung sections by immunohistochemistry. p85-PARP was significantly higher in 1 -4 dPi lung sections compared to Sham controls, peaked at 4 dPi and decreasing significantly by 7 dPi (Figure 4A). This trend in apoptosis in the Acute model was coordinate with the changes in DR4 and DR5 expression (Figure 4B). * p < 0.05 compared to Sham and †p < 0.05 compared to 4 dPi.

Article Snippet: Polyclonal rabbit anti-DR4 and -DR5 antibodies (Cell Sciences Inc, MA) and rabbit anti- PARP p85 fragment antibody (Promega, MA) were used along with normal Rabbit IgG as negative control to measure receptor expression and apoptosis respectively. p85-PARP antibody is specific for the p85 fragment of PARP generated by caspase cleavage and provides a reliable measure of in situ apoptosis [ ].

Techniques: Infection, Expressing, Immunohistochemistry

Journal: Respiratory Research

Article Title: Apoptosis of viral-infected airway epithelial cells limit viral production and is altered by corticosteroid exposure

doi: 10.1186/1465-9921-7-78

Figure Lengend Snippet: Guinea pig AEC apoptosis and DR expression as detected by Immunohistochemistry of the Chronic model of Guinea pig viral infection and airway inflammation Semi-quantitative scoring was utilized to determine the expression of p85-PARP, DR4 and DR5 in the Guinea pig lung sections by immunohistochemistry. Significant reduction in the detection of p85-PARP for Ad5+OVA+Bud group (** p < 0.005) was observed when compared to Ad5 alone group (Figure 7A). However, DR4/DR5 expression for Ad5+OVA+Bud group was higher compared to Ad5 alone group * p < 0.05 (Figure 7B).

Article Snippet: Polyclonal rabbit anti-DR4 and -DR5 antibodies (Cell Sciences Inc, MA) and rabbit anti- PARP p85 fragment antibody (Promega, MA) were used along with normal Rabbit IgG as negative control to measure receptor expression and apoptosis respectively. p85-PARP antibody is specific for the p85 fragment of PARP generated by caspase cleavage and provides a reliable measure of in situ apoptosis [ ].

Techniques: Expressing, Immunohistochemistry, Infection